methylenetetrahydrofolate reductase c677t and a1298c polymorphism in iranian women with idiopathic recurrent pregnancy losses

Authors

elham yousefian department of midwifery, islamic azad university falavarjan branch, isfahan, ir iran; department of midwifery, islamic azad university falavarjan branch, isfahan, ir iran. tel: +98-3123120136

mohammad taghi kardi department of biology, university of isfahan, isfahan, ir iran

azra allahveisi department of anatomy, faculty of medicine, kurdistan university of medical sciences, sannandaj, ir iran

abstract

background recurrent pregnancy loss (rpl) is a serious problem for pregnancy. there is evidence that vascular complications play a principal role in rpl. methylenetetrahydrofolate reductase (mthfr) is a key enzyme in folate metabolism. polymorphisms (c677t, a1298c) of mthfr gene are associated with decreased mthfr activity. objectives the aim of this study was to determine the association between mthfr polymorphisms (c677t and a1298c) and recurrent pregnancy loss (rpl) in iranian women. materials and methods in this case-control study, blood samples were obtained from patients who had three or more consecutive pregnancy losses before the 22nd week of pregnancy (n = 204). the control group consisted of 116 age-matched women with at least one alive child and without any history of pregnancy loss or other gestational complications (n = 116). following dna extraction, samples were tested for mthfr c677t and a1298c polymorphisms using the reverse hybridization method. results the prevalence of 677tt mutation was 8.8% (18/204) in the patient group and 8.6% (10/116) in the control group (p = 0.434). the prevalence of 1298cc mutation was 12.3 % (25/204) in the patient group and 8% (9/116) in the control group (p = 0.155). investigation of the distributions of various genotypes of mthfr c677t and a1298c did not indicate a significant difference between patients with rpl and healthy control subjects. conclusions the results suggest that mthfr mutations might not be associated with rpl in the examined population.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Methylenetetrahydrofolate Reductase C677T and A1298C Polymorphism in Iranian Women With Idiopathic Recurrent Pregnancy Losses

BACKGROUND Recurrent pregnancy loss (RPL) is a serious problem for pregnancy. There is evidence that vascular complications play a principal role in RPL. Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in folate metabolism. Polymorphisms (C677T, A1298C) of MTHFR gene are associated with decreased MTHFR activity. OBJECTIVES The aim of this study was to determine the association bet...

full text

C677T and A1298C Mutations in the Methylenetetrahydrofolate Reductase Gene in Patients with Recurrent Abortion from the Iranian Azeri Turkish

Background To assess whether the C677T and A1298C mutations in the methylenetetrahydrofolate reductase (MTHER) gene are associated with recurrent abortion (RA), we determined the frequencies of the T677 and C1298 mutations in patients and controls. MaterialsAndMethods Mutations were determined by a RFLP-PCR method in 53 patients and 61 matched controls. Results The frequencies of T alleles were...

full text

Evaluation of the Association between the C677T Polymorphism of Methylenetetrahydrofolate Reductase Gene and Recurrent Spontaneous Abortion

Introduction: One factor known to cause thrombophilia in women with unexplained recurrent spontaneous abortion (RSA) is C677T polymorphism of methylenetetrahydrofolate reductase gene. This study aimed to determine the association between RSA and MTHFR C677T polymorphism in Iranian patients. Methods: In this case-control study, 30 patients with previous history of two or more consecutive unexpla...

full text

Association between the A1298C Polymorphism of the Methylenetetrahydrofolate Reductase Gene and Recurrent Spontaneous Abortion

Introduction: A factor known to cause thrombophilia in women with recurrent pregnancy loss (RPL) is the A1298C polymorphism of methylenetetrahydrofolate reductase gene (MTHFR). This study aimed to determine the association between RPL and this polymorphism in Iranian patients. Methods: In this case-control study, 30 patients with a previous history of two or more consecutive unexplained abortio...

full text

Methylenetetrahydrofolate reductase C677T polymorphism and cognitive function in older women.

Homocysteine may play a causal role in cognitive decline. The authors analyzed the 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T genotype, a correlate of plasma homocysteine levels, among 6,653 participants in the Study of Osteoporotic Fractures, a community-based, prospective cohort study of older women in four US states. During the years 1986-1998, the authors assessed whether the di...

full text

Methylenetetrahydrofolate Reductase C677T and A1298C Mutations in Women with Recurrent Spontaneous Abortions in the Northwest of Iran

Introduction. Recurrent spontaneous abortion (RSA) is a significant obstetrical complication that may occur during pregnancy. Various studies in recent years have indicated that two common mutations (C677T and A1298C) of the methylenetetrahydrofolate reductase (MTHFR) gene are risk factor for RSA. This study was carried out to determine the influence of (C677T and A1298C) of the methylenetetrah...

full text

My Resources

Save resource for easier access later


Journal title:
iranian red crescent medical journal

جلد ۱۶، شماره ۷، صفحات ۰-۰

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023